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微管蛋白β tubulin/Tubulin β抗體
  • 產(chǎn)品貨號:
    BN40630R
  • 中文名稱:
    微管蛋白β tubulin/Tubulin β抗體
  • 英文名稱:
    Rabbit anti-Beta tubulin Polyclonal antibody
  • 品牌:
    Biorigin
  • 貨號

    產(chǎn)品規(guī)格

    售價

    備注

  • BN40630R-100ul

    100ul

    ¥2360.00

    交叉反應(yīng):Human,Mouse,Rat(predicted:Pig,Rabbit) 推薦應(yīng)用:WB,IHC-P,IHC-F,IF,Flow-Cyt

  • BN40630R-200ul

    200ul

    ¥3490.00

    交叉反應(yīng):Human,Mouse,Rat(predicted:Pig,Rabbit) 推薦應(yīng)用:WB,IHC-P,IHC-F,IF,Flow-Cyt

產(chǎn)品描述

英文名稱Beta tubulin
中文名稱微管蛋白β tubulin/Tubulin β抗體
別    名Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.  
研究領(lǐng)域細胞生物  免疫學(xué)  神經(jīng)生物學(xué)  細胞骨架  
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng)Human, Mouse, Rat,  (predicted: Pig, Rabbit, )
產(chǎn)品應(yīng)用WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
細胞定位細胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Beta tubulin:61-160/444 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit:
Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity).

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications:
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity:
Belongs to the tubulin family.

SWISS:
P07437

Gene ID:
203068

Database links:

Entrez Gene: 203068 Human

Omim: 191130 Human

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

結(jié)構(gòu)蛋白(Structural Proteins)
tubulin是一種大量存在于哺乳動物腦組織中的微管亞基蛋白,在結(jié)構(gòu)上是由兩個極為相近的α和β亞基組成的二聚體、多聚體形成微管細絲,是微管的主要成分。
微管蛋白是球形分子, 有兩種類型:α微管蛋白(α-tubulin)貨號:bs-0195R和β微管蛋白(β-tubulin), 這兩種微管蛋白具有相似的三維結(jié)構(gòu), 能夠緊密地結(jié)合成二聚體, 作為微管組裝的亞基。 α亞基由450個氨基酸組成, β亞基是由455個氨基酸組成, 這兩種亞基有35~40%的氨基酸序列同源, 表明編碼它們的基因可能是由同一原始祖先演變而來.




















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